Abstract: To observe the immunotherapeutic effects of dendritic cell vaccine pulsed with rat pancreatic cancer cell lysate on rat pancreatic cancer. Methods: Rat pancreatic cancer cells were injected into the pancreatic capsule of SD rats. The formed tumor was chopped and implanted subcutaneously into the axillary of rats to establish rat model of pancreatic cancer. Rat pancreatic cells were repeatedly frozen and thawed 4 times,and then were extracted from lysate cultured for 72 hours with the SD rat bone marrow-derived DC to produce DC vaccines. According to DC pulse, 5 groups were set ( DC vaccine group, DC group, control group and lysate group ), with 3 rats in each group. The tumor length and diameter were measured every two days, 8 times in total and the survival time of rats were observed. Results: The tumor size in DC vaccine group was decreased, and the tumor size in DC group, lysate group, and control group showed an increasing trend. Statistically significance in tumor size between DC vaccine group and control group was found ( P < 0.05 ). The difference in tumor size among DC group, lysate group and control group was not significant ( P > 0.05 ). The difference in tumor size among DC vaccine group, DC group, and lysates group was significant ( P < 0.05 ). The survival of rats in DC vaccine group was( 55.6±9.4 )d,significantly longer than that in other groups( P < 0.0 1 ). Conclusion: Rat pancreatic cancer cell lysate-pulsed dendrtic cell vaccine may induce a specific and effective immune response against pancreatic cancer cells implanted in rats.